Award details

High resolution imaging of intracellular Ca2+ in plants

Reference	ICS00797
Principal Investigator / Supervisor	Professor Peter Shaw
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	Cell and Develop Biology
Funding type	Research
Value (£)	8,775
Status	Completed
Type	Research Grant
Start date	01/05/1994
End date	01/05/1997
Duration	36 months

Abstract

The aim of this project is to establish the technology for imaging of calcium and other plant cell signalling components by real time confocal laser scanning ratio microscopy at JIC. This technology will be used to investigate and elucidate mechanisms of intracellular signalling in plant cells following a 4 point plan: 1. The effect of microinjection of signalling components on Ca2+ fluxes across endomembranes, the cytosol and the plasma membrane will be investigated. Particular attention will be given to spatial aspects of CA2+ mobilisation; 2. The role of Ca2+ in red-light induced responses in wheat protoplasts will be studied; 3. Temporal and spatial aspects of ABA-induced cytosolic Ca2+ fluxes in stomatal guard cells will be further characterised; 4. The role of Ca2+ in cytokinin responses in moss will be studied. Special attention will be given to the possibility of Ca2+ fluxes in the vicinity of, and across, the nuclear envelope and of Ca2+-H+ oscillatory behaviour in parts 2-4 of the project.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Intracellular Signalling (ICS) [1986]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search