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Mutagenesis of the signal transduction pathway responsible for the induction of plant defence genes by salicylic acid
Reference
ICS00764
Principal Investigator / Supervisor
Professor John Draper
Co-Investigators /
Co-Supervisors
Institution
Aberystwyth University
Department
Inst of Biological, Environ & Rural Sci
Funding type
Research
Value (£)
253,177
Status
Completed
Type
Research Grant
Start date
01/03/1993
End date
01/08/1998
Duration
65 months
Abstract
AoPR1 is a plant 'defence' gene that is strongly induced by exogenously-supplied salicylic acid and the project aims to investigate the signal transduction mechanism responsible for gene activation. Homozygous Arabidopsis lines containing stable, marked Ds elements, a functional AoPR1-GUS reporter and a AoPR1 negative selection/screenable reporter gene construct will be made. These will be crossed with plants containing a microspore-specific Ac transposes. F2 seed and their future progeny will be screened for salicylate signal transduction mutants Transposon insertion mutants will be stabilised by segregating out the transposes helper. The DNA flanking the inserts will be cloned by inverse PCR and used to isolate wild type genes which may then be analysed as candidate genes involved in signal transduction pathways responsible for activation of plant defence genes.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Intracellular Signalling (ICS) [1986]
Funding Scheme
X – not Funded via a specific Funding Scheme
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