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Integrin-associated cytoskeletal proteins: structural and functional studies

Reference	ICR07623
Principal Investigator / Supervisor	Professor David Critchley
Co-Investigators / Co-Supervisors	Professor Clive Bagshaw, Dr Robert Liddington
Institution	University of Leicester
Department	Biochemistry
Funding type	Research
Value (£)	132,772
Status	Completed
Type	Research Grant
Start date	01/12/1997
End date	28/02/2001
Duration	39 months

Abstract

Talin and vinculin are cytoskeletal proteins thought to link integrins to F-actin, a link essential to integrin-mediated cell adhesion and signal transduction. Sub-domains of these proteins will be expressed in Escherichia coli and the structures of the purified proteins determined by crystallography. The kinetics of the interactions between these proteins will be characterised using solid phase and solution assays, and the possibility that the vinculin and actin binding sites in talin are regulated by an intramolecular interaction within the talin molecule explored. In vitro assays will be developed to study the assembly of the cytoskeletal complex either using isolated focal adhesions stripped of actin or using immobilised talin as a nucleating protein.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Integration of Cellular Responses (ICR) [1996]
Funding Scheme	X – not Funded via a specific Funding Scheme

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