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Molecular integration of cellular responses involved in polarity growth and cell volume control in Fucus zygotes

Reference	ICR07562
Principal Investigator / Supervisor	Professor Colin Brownlee
Co-Investigators / Co-Supervisors	Professor Tony Trewavas
Institution	Marine Biological Association
Department	The Laboratory
Funding type	Research
Value (£)	178,377
Status	Completed
Type	Research Grant
Start date	01/09/1997
End date	01/09/2000
Duration	36 months

Abstract

Localised Ca2+ signals underlie both polarised growth and cell volume regulation in the Fucus zygote. We will investigate how they are generated and how they elicit different early downstream responses in the same cell. We propose that: (1) Different Ca2+ channels underlie signals involved in establishment of polarity, polar cell growth and volume regulation. (2) Activation of specific Ca2+ channels can lead to different signal patterns involving Ca2+ influx and intracellular release. (3) Different signal patterns effect different early downstream responses. Confocal imaging and line scans in intact cells and simultaneous patch clamp and confocal imaging of laser-derived protoplasts will be used for detailed studies of the Ca2+ dynamics in response to direct manipulation of specific Ca2+ channels. We will also determine patterns of calmodulin activation, the involvement of membrane recycling and ion efflux channels in each response, and the role of membrane cell wall focal adhesions in regulation of localised Ca2+ signals.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Integration of Cellular Responses (ICR) [1996]
Funding Scheme	X – not Funded via a specific Funding Scheme

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