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Definition of the regulatory domains of a PtdIns4P 5-kinase Pipkin C and the role of PtdIns(45)P2 as a potential second messenger

ReferenceICR07552
Principal Investigator / Supervisor Professor Robin Irvine
Co-Investigators /
Co-Supervisors
Institution University of Cambridge
DepartmentPharmacology
Funding typeResearch
Value (£) 138,743
StatusCompleted
TypeResearch Grant
Start date 03/11/1997
End date 30/06/2001
Duration42 months

Abstract

We have purified, sequenced and cloned a novel PtdIns4P 5-kinase, Pipkin C, which is regulated by multiple signalling pathways. The enzyme which contains two putative SH3 domain binding motifs is regulated by dephosphorylation/phosphorylation, by interaction with at least two small molecular weight G-proteins (SMGs), namely Rac and Rho, and by integrin-mediated signals. We aim to identify the domains involved with these different modes of regulation and to use this information to develop tools to compromise them and so define the in vivo role of PtdIns(4,5)P2 in cellular regulation.

Summary

unavailable
Committee Closed Committee - Biochemistry & Cell Biology (BCB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Integration of Cellular Responses (ICR) [1996]
Funding SchemeX – not Funded via a specific Funding Scheme
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