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The mechanism of capacitative calcium entry

Reference	ICR07498
Principal Investigator / Supervisor	Professor Sir Michael Berridge
Co-Investigators / Co-Supervisors	Dr Martin Bootman
Institution	Babraham Institute
Department	Babraham Institute Department
Funding type	Research
Value (£)	183,181
Status	Completed
Type	Research Grant
Start date	01/10/1997
End date	01/10/2000
Duration	36 months

Abstract

This proposed study aims to investigate the mechanism by which depleted stores stimulate Ca2+ entry through store-operated channels (SOCs). Building on previous evidence that vertebrate homologues of the Drosophila trp gene may function as SOCs, an initial objective will be to use standard molecular (PCR, expression and deletion analysis) and physiological (Ca2+ imaging and patch-clamp) techniques to establish the presence and function of SOCs in our model systems (Xenopus oocytes, HeLa, CHO and Sf9 cells). Similar techniques, together with e.m. immunocytochemistry, will examine the proposal that the type 3 InsP3 receptors are directly linked to these SOCs through a conformational coupling mechanism.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Integration of Cellular Responses (ICR) [1996]
Funding Scheme	X – not Funded via a specific Funding Scheme

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