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Functional and structural analyses of the adaptor molecule Shc in IL-3 dependent signal transduction
Reference
ICR07479
Principal Investigator / Supervisor
Professor Melanie Welham
Co-Investigators /
Co-Supervisors
Institution
University of Bath
Department
Pharmacy and Pharmacology
Funding type
Research
Value (£)
79,188
Status
Completed
Type
Research Grant
Start date
01/03/1997
End date
01/03/1999
Duration
24 months
Abstract
The aim of this study is to characterise the functions of Shc in integrating intracellular signals generated by IL-3. This approach will use a combination of genetic, cellular, biochemical and structural techniques. Shc mutants, targetting the PTB, CHI and SH2 domains and Y317 will be constructed and expressed in IL-3 dependent hemopoietic cells, using the tetracycline controlled gene expression system. Functional analyses of transfectants will include examination of IL-3 induced cellular proliferation; cell cycle; activation of the ras/MAP kinase, PI3-kinase and Jak-STAT pathways and inositol lipid metabolism amongst others. The protein-interactions mediated by Shc will be assessed in transfectants and using biochemical techniques. Ultimately, we want to relate Shc function to its structure and will embark on structural studies with the full length Shc molecule.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Integration of Cellular Responses (ICR) [1996]
Funding Scheme
X – not Funded via a specific Funding Scheme
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