Award details

Development of a human 2.4 Mb minichromosome as a vector for gene transfer

Reference	GTH12584
Principal Investigator / Supervisor	Dr Clare Huxley
Co-Investigators / Co-Supervisors	Professor William Colledge, Dr Christine Farr
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	166,416
Status	Completed
Type	Research Grant
Start date	01/08/2000
End date	01/08/2003
Duration	36 months

Abstract

This proposal centres on the development of a 2.4 Mb human X centromere-based minichromosome as a vector for gene transfer into mice and for gene therapy applications. A system for retrofitting the minichromosome with large DNA inserts (PACs or BACs) using the Cre-lox system will be assessed. Microcell mediated chromosome transfer will be used to determine whether the minichromosome can be stably maintained in untransformed human somatic cells and whether it can be transmitted, both mitotically and meiotically, in another mammalian species; the mouse. The embryonic stem cell route will be used to generate transchromosomal animals. Finally, the expression of genes present on the minichromosome (endogenous (ZXDA) and introduced (HPRT and beta-globin)) will be investigated, both in cultured cells and in chimeric and germline transchromosomal mice. (Joint application with grant 28/GTH12583)

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Gene Technologies Underpinning Healthcare (GTH) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

GTH12583 Development of a human 2.4 Mb human minichromosome as a gene transfer vector

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