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Optimising axonal delivery of therapeutic genes in the spinal cord using polycationic vectors
Reference
GTH12577
Principal Investigator / Supervisor
Professor Ann Logan
Co-Investigators /
Co-Supervisors
Professor Martin Berry
,
Professor Leonard Seymour
Institution
University of Birmingham
Department
Medical Sciences - Medicine
Funding type
Research
Value (£)
192,389
Status
Completed
Type
Research Grant
Start date
01/11/1999
End date
01/11/2002
Duration
36 months
Abstract
The project aims to optimise therapeutic transgene targeting and duration of expression using targeted polycationic gene delivery vectors in an ascending sensory tract of the spinal cord. Accordingly, using labelled DNA to monitor DNA trafficking and RT-PCR/in situ hybridisation/immunohistochemistry/Western blotting to monitor transgene mRNA/protein expression, we will investigate in dorsal root ganglia in vitro and in vivo: 1) DNA targeting and persistence of transgene expression using neuron-specific promoters; 2) DNA targeting using CTb, TT-HC and FGF-2 targeting ligands; 3) viral methods of enhancing longevity of transgene expression.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Gene Technologies Underpinning Healthcare (GTH) [1999]
Funding Scheme
X – not Funded via a specific Funding Scheme
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