Award details

Assessment of the nuclear reprogramming capacity of ES cytoplasm

Reference	GTH12560
Principal Investigator / Supervisor	Dr James McWhir
Co-Investigators / Co-Supervisors	Dr Michael Clinton, Professor Azim Surani
Institution	The Roslin Institute
Department	Gene Expression and Development
Funding type	Research
Value (£)	289,636
Status	Completed
Type	Research Grant
Start date	26/01/2000
End date	26/05/2003
Duration	40 months

Abstract

Adult somatic nuclei are capable of directing normal development following nuclear transfer. This project is to determine if ES cytoplasm also has nuclear reprogramming activity which can be harnessed to generate ES cell lines directly from somatic cells. If successful, this approach provides an opportunity to derive isogenic ES cell lines for tissue transplantation therapy and gene therapy. The approach is to generate fusions of ES cells with somatic cells to determine if the ES chromosomes are required for maintenance of the ES phenotype either by fusion of somatic cells with ES cytoplasts or by fusion with ES cells in which the chromosomes have been treated to prevent their replication and segregation.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Gene Technologies Underpinning Healthcare (GTH) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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