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Award details
Mechanism and exploitation of ubiquitous chromatin opening elements - UCOEs
Reference
GTH12532
Principal Investigator / Supervisor
Dr Michael Antoniou
Co-Investigators /
Co-Supervisors
Institution
King's College London
Department
Medical & Molecular Genetics SM
Funding type
Research
Value (£)
335,086
Status
Completed
Type
Research Grant
Start date
01/08/2000
End date
01/11/2004
Duration
51 months
Abstract
The human TBP and hnRNP-A2 loci have been found to possess ubiquitous chromatin opening elements (UCOEs) that appear to consist of two closely linked, divergently transcribed promoters associated with a methylation-free, CpG island. The aim of this proposal is to investigate the mechanisms of UCOE function and to optimise vectors for gene therapy and protein production in cultured cells. Functional analysis in stable transfected cells and mouse models will be used to obtain mechanistic insight and assess efficacy in elevated and prolonged gene expression. UCOE-based vectors will give stable and high-level transgene expression in vivo and in vitro in all cell types thereby overcoming a major problem faced by the biotechnology industry.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Gene Technologies Underpinning Healthcare (GTH) [1999]
Funding Scheme
X – not Funded via a specific Funding Scheme
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