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Reprogramming a somatic nucleus to totipotency: developmental and epigenetic analyses
Reference
GTH12527
Principal Investigator / Supervisor
Professor Azim Surani
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Gurdon Institute
Funding type
Research
Value (£)
150,512
Status
Completed
Type
Research Grant
Start date
01/04/2000
End date
01/08/2003
Duration
40 months
Abstract
Somatic nuclei will be transplanted into enucleated mouse oocytes to investigate the mechanism and consequences of reprogramming of the somatic nucleus. The extent of embryonic development and its relationship to the epigenetic modifications of the somatic nucleus will be examined. Development of embryonic and extraembryonic tissues will be assessed independently by blastocyst reconstitution and in chimeric embryos. Embryonic stem cells and trophectoderm cell lines will be established for detailed phenotypic and molecular analysis. The erasure or heritability of epigenetic modifications in the donor somatic nucleus (that is highly methylated initially), will be monitored by DNA methylation analysis of imprinted and other loci. Both embryonic and trophectoderm tissues will be examined since they are fundamentally dissimilar.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Gene Technologies Underpinning Healthcare (GTH) [1999]
Funding Scheme
X – not Funded via a specific Funding Scheme
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