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Controlled gene specific methylation by in vivo expression of targeted DNA methyltransferases: A novel gene therapy approach

Reference	GTH12521
Principal Investigator / Supervisor	Dr Kevin Ford
Co-Investigators / Co-Supervisors
Institution	King's College London
Department	GKT School of Medicine
Funding type	Research
Value (£)	178,088
Status	Completed
Type	Research Grant
Start date	01/01/2000
End date	01/10/2003
Duration	45 months

Abstract

The inheritable nature of DNA methylation patterns within the cell, coupled to the inhibitory effect of methylation on gene transcription, offers a novel 'single-shot' gene therapy approach, through the application of sequence targeted DNA cytosine methyltransferases. We have constructed a number of enzymes capable of specific methylation of predetermined sequences and we now propose to evaluate these enzymes for their in vivo ability to both deliver a methylation signal at the desired locus and repress transcription via a methylation mediated pathway. We also describe a number of experiments, where for the first time, the effects of variance in methylation density on transcription, chromatin structure and methylation spread, can be evaluated at a single defined site, within a constant model system.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Gene Technologies Underpinning Healthcare (GTH) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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