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Cloning and epigenetic reprogramming in the early mouse embryo

Reference	GTH12511
Principal Investigator / Supervisor	Professor Wolf Reik
Co-Investigators / Co-Supervisors	Dr Wendy Dean
Institution	Babraham Institute
Department	Developmental Genetics and Imprinting
Funding type	Research
Value (£)	192,524
Status	Completed
Type	Research Grant
Start date	01/11/1999
End date	01/11/2002
Duration	36 months

Abstract

The recent advent of cloning in mammals promises to have a huge impact on our basic understanding of totipotency and on applications in therapy and healthcare. However currently the techniques are inefficient and the process of nuclear reprogramming is not understood. Here we propose to look at major events of epigenetic reprogramming in somatic cell genomes introduced into mouse oocytes. In particular, methylation reprogramming in imprinted and non-imprinted genes will be investigated using the highly sensitive bisulphite sequencing technique. Features of reprogramming will be related to developmental potential of cloned embryos. This work will provide mechanistic insights into cloning, and improve prospects for applications.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Gene Technologies Underpinning Healthcare (GTH) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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