BBSRC Portfolio Analyser
Award details
Gene targeting in mucosal T cells
Reference
GTH12498
Principal Investigator / Supervisor
Dr Peter Kilshaw
Co-Investigators /
Co-Supervisors
Institution
Babraham Institute
Department
Babraham Institute Department
Funding type
Research
Value (£)
165,584
Status
Completed
Type
Research Grant
Start date
23/04/2000
End date
22/10/2003
Duration
42 months
Abstract
The alphaE integrin gene is transcribed only in mucosal T cells and mucosal dendritic antigen presenting cells. We propose to exploit the transcription control elements to direct expression of E. coli nitroreductase (NTR) in transgenic mice by introducing the NTR gene into the first exon of alphaE by homologous recombination. NTR has the capacity to convert the prodrug CB1954 to a cytotoxin. Thus, when mice are injected with CB1954, NTR+ve cells will be killed in a highly specific manner. The effect of ablating this compartment of the mucosal immune system will be tested in mouse models of inflammatory bowel disease, asthma, oral tolerance and other parameters of mucosal immunity.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Gene Technologies Underpinning Healthcare (GTH) [1999]
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search