Award details

3D analysis of transgene integration and expression in wheat

ReferenceGM114213
Principal Investigator / Supervisor Professor Peter Shaw
Co-Investigators /
Co-Supervisors
Institution John Innes Centre
DepartmentCell and Develop Biology
Funding typeResearch
Value (£) 267,876
StatusCompleted
TypeResearch Grant
Start date 02/07/2001
End date 02/07/2004
Duration36 months

Abstract

We have recently shown that multiple transgenes integrated into the wheat genome can be separated by large regions (several Mb) of genomic sequence, or can even be on opposite chromosome arms. However, in interphase these multiple sites are generally recruited to or organized at a single site in the interphase chromosome territories. This proposal is aimed at understanding the mechanism underlying the interphase organization; does it reflect a highly conserved interphase chromosome architecture, or is it the result of incorporating novel transgenes and associated sequences into the genome? Such ectopic interactions between multiple transgenes have implications for gene expression, transcriptional silencing, and meiotic homologue pairing.

Summary

unavailable
Committee Closed Committee - Agri-food (AF)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Gene Flow in Plants and Microorganisms (GM1) [2000]
Funding SchemeX – not Funded via a specific Funding Scheme
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