Award details

Evaluation of recombinant chromosomal gene transfer from plant genomes to indigenous soil bacteria

Reference	GM114168
Principal Investigator / Supervisor	Dr Martin Day
Co-Investigators / Co-Supervisors	Professor John Fry, Professor Hilary Joan Rogers
Institution	Cardiff University
Department	School of Biosciences
Funding type	Research
Value (£)	161,352
Status	Completed
Type	Research Grant
Start date	01/09/2001
End date	01/09/2004
Duration	36 months

Abstract

There is still a lack of data on the frequency of transgene movement from plants to soil bacteria in the field, or on the range of potential bacterial recipients and factors influencing transformation frequency. To improve chances of detectable levels of transformation, we will use a plant system containing a high ratio of GM to non-GM DNA, and transgenes which favour homologous recombination both to the marked strains and to native soil bacteria. Using this system we will test parameters which influence efficiency of transformation and survival of transformed bacteria in microcosms. We will then use plant transgenic material in microcosm experiments to assess levels and range of transformable bacteria as well as their survival.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Gene Flow in Plants and Microorganisms (GM1) [2000]
Funding Scheme	X – not Funded via a specific Funding Scheme

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