Award details

Gene targeting and DNA double strand break repair in Arabidopsis

Reference	GM114142
Principal Investigator / Supervisor	Dr Clifford Bray
Co-Investigators / Co-Supervisors	Professor Simon Turner, Dr Christopher West
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	250,604
Status	Completed
Type	Research Grant
Start date	01/02/2001
End date	01/02/2004
Duration	36 months

Abstract

The relative rates of gene replacement versus random integration events are governed by the enzymology of the respective pathways. Recently we have cloned several key genes of the non-homologous end joining (NHEJ) pathway responsible for random transgene incorporation into the plant genome. We propose to employ a combined biochemical and molecular genetic approach to (i) enhance our fundamental knowledge of the mechanism of NHEJ in higher plants, (ii) identify those key components of the NHEJ complex which do not affect homologous recombination (iii) isolate and characterise T-DNA tagged mutants in specific NHEJ genes of interest (iv) assess the effects of these mutations on transformation efficiency and gene targeting in Arabidopsis.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Gene Flow in Plants and Microorganisms (GM1) [2000]
Funding Scheme	X – not Funded via a specific Funding Scheme

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