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The partitioning of nitrate assimilation in higher plants as an adaption to climatic conditions
Reference
GER00648
Principal Investigator / Supervisor
Professor Brian Gordon Forde
Co-Investigators /
Co-Supervisors
Institution
Rothamsted Research
Department
Biological Chemistry & Crop Protection
Funding type
Research
Value (£)
23,776
Status
Completed
Type
Research Grant
Start date
01/06/1993
End date
01/06/1997
Duration
48 months
Abstract
There is strong circumstantial evidence that differential partitioning of nitrate assimilation is a significant factor in adaptation to growth under different environmental conditions. We propose to investigate this using genetic manipulation to generate mutants of Lotus japonicus and tomato, in which the partitioning of nitrate assimilation is altered. L. japonicus will be transformed with an antisense nitrate reductase gene under the control of a root-specific promotor, to reduce nitrate reductase activity in the root. Tomato will be transformed with genes designed to overexpress nitrate reductase and nitrate reductase in the root. The effect of the manipulations will be assessed by analysis of control and transgenic lines grown under various conditions of temperature and light intensity.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Global Environmental Response (GER) [1986]
Funding Scheme
X – not Funded via a specific Funding Scheme
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