Award details

Function search in Arabidopsis by saturation transposon mutagenesis

Reference	GAT09113
Principal Investigator / Supervisor	Professor Jonathan Jones
Co-Investigators / Co-Supervisors
Institution	University of East Anglia
Department	Sainsbury Laboratory
Funding type	Research
Value (£)	120,988
Status	Completed
Type	Research Grant
Start date	07/01/1998
End date	07/04/2000
Duration	27 months

Abstract

Over the next 3 years, 70-80% of the Arabidopsis gene sequences will be discovered. Many of these genes are of unknown function. One way to discover their function is by generating a large library of lines carrying transpositions, with subsequent screening by PCR for insertions in defined gene sequences. With sufficient insertions (approx 60,000) the probability of obtaining an insertion can be high (approx. 80%). A Seqenced INSertion site (SINS) database containing 5000 sequences will be established to facilitate detection of interesting insertions. We will use this resource to knock out and characterise genes putatively involved in resistance gene function. This resource will be made publicly available through the Nottingham Arabidopsis Stock Centre.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Genome Analysis of Agriculturally Important Traits (GAT) [1997-1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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