Award details

Development of methods for the identification and selection of mapped genes targeting a recently mapped resistance gene in chickens

Reference	GAT09084
Principal Investigator / Supervisor	Dr N Bumstead
Co-Investigators / Co-Supervisors	Professor Stephen Kemp
Institution	The Pirbright Institute
Department	Div of Molecular Biology
Funding type	Research
Value (£)	237,576
Status	Completed
Type	Research Grant
Start date	01/11/1998
End date	01/11/2001
Duration	36 months

Abstract

This project will investigate and develop methods for the identification and selection of mapped genes, focusing on a novel salmonellosis-resistance gene which we have recently mapped to chicken chromosome 5. Additional markers in the region of this gene will be generated through construction of a chromosome 5 flow-sorted library and through Representational Difference Analysis: these markers will be used to precisely locate the resistance gene and to isolate YAC clones containing the gene. Genes within these YACs will be identified by exon trapping, ORF trapping and sequence skimming, and the identity of the gene confirmed by its pattern of expression and sequence comparisons. At the same time methods for selection for resistant animals in commercial flocks using gel-free systems will be investigated in collaboration with Ross Breeders UK Ltd.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Genome Analysis of Agriculturally Important Traits (GAT) [1997-1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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