Award details

Radiation hybrid mapping: a somatic cell genetics approach to plant genome analysis

Reference	GAT09065
Principal Investigator / Supervisor	Dr Gordon Machray
Co-Investigators / Co-Supervisors	Professor John Snape
Institution	The James Hutton Institute
Department	Cell and Molecular Genetics Department
Funding type	Research
Value (£)	140,627
Status	Completed
Type	Research Grant
Start date	13/07/1998
End date	13/11/2001
Duration	40 months

Abstract

We wish to test the hypothesis that hybrid plant cells produced by the fusion of an irradiated donor cell can be used to produce information on the genome composition of plants. Protoplasts derived from herbicide resistant barley cells will be irradiated and fused with recipient potato protoplasts. The resultant somatic hybrids or radiation hybrids (RH) will be cultured in selective media. Each RH cell line will be characterised at the molecular (microsatellite) and cytogenetic (FISH) levels to determine the relationship between barley retention frequency and irradiation dosage. Data emerging from this study will provide fundamental information on the feasibility of using this approach to localise and order genes in plant genomes.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Genome Analysis of Agriculturally Important Traits (GAT) [1997-1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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