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G protein coupled receptors in C. elegans: finding the ligands

Reference	GAN13124
Principal Investigator / Supervisor	Dr Christopher Ford
Co-Investigators / Co-Supervisors	Professor Lindy Holden-Dye
Institution	University of Sussex
Department	Biology and Environmental Science
Funding type	Research
Value (£)	428,631
Status	Completed
Type	Research Grant
Start date	01/10/2000
End date	01/05/2004
Duration	43 months

Abstract

The sequencing of the C. elegans genome has revealed 140 non-olfactory G protein-coupled receptors (GPCRs) (www.sanger.ac.uk/Projects/C elegans). At least half of these are likely to be neuropeptide receptors rather than receptors for classical transmitters. Analysis of the sequencing data also shows 30 putative neuropeptide precursor genes most of which encode multiple peptides. The aim of this study is to systematically delete by mutagenesis, inactivate by RNAi 20 neuropeptide GPCR genes and identify the peptides which act as ligands for these GPCRs. This will be the first systematic genomic approach to determine which receptor binds to which ligand and forms the first part of a long term project to characterise all receptor:ligand pairs in C. elegans. The mutants will be further analysed to determine the role of neuropeptide GPCRs and their peptide ligands in C. elegans physiology and behaviour.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Genomics in Animal Function Initiative (GAN) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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