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Identification and functional analysis of synaptic transmission dependent genes in Drosophila

ReferenceGAN13123
Principal Investigator / Supervisor Professor Michael Bate
Co-Investigators /
Co-Supervisors
Institution University of Cambridge
DepartmentZoology
Funding typeResearch
Value (£) 285,824
StatusCompleted
TypeResearch Grant
Start date 01/05/2000
End date 01/08/2003
Duration39 months

Abstract

Synaptic transmission is required for functional maturation of the CNS and to induce gene transcription involved in neuronal plasticity. Our aim is to use Drosophila to identify genes that are transcriptionally regulated by synaptic transmission during development. Differential screening and cDNA microarrays will be used to compare gene expression in normal embryos and embryos with no synaptic transmission. RNA interference knockouts of confirmed positive clones will be assessed initially with a rapid behavioural and anatomical screen. Detailed electrophysiological and structural investigation will identify those clones corresponding to genes required for the functional maturation of the CNS. These genes will be mapped and sequenced.

Summary

unavailable
Committee Closed Committee - Animal Sciences (AS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Genomics in Animal Function Initiative (GAN) [1998]
Funding SchemeX – not Funded via a specific Funding Scheme
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