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Neuropeptide Y and Y1 receptor knock-outs: functional consequences in the gastrointestinal tract

Reference	GAN13101
Principal Investigator / Supervisor	Professor Helen Cox
Co-Investigators / Co-Supervisors
Institution	King's College London
Department	GKT School of Biomedical Sciences
Funding type	Research
Value (£)	181,948
Status	Completed
Type	Research Grant
Start date	03/06/2000
End date	03/12/2003
Duration	42 months

Abstract

This project will investigate the functional consequences of unconditional knock-out of the neuropeptide Y (NPY) gene or the NPY Y1 receptor in gastrointestinal mucosae from homozygote mutant mice. We have shown NPY, peptide YY (PYY) and pancreatic polypeptide (PP) to be potent antisecretory agents in normal mouse colon in vitro and that in NPY-/- colon mucosa there is a potential increase in sensitivity to NPY and PYY, but not PP or the alpha2- agonist UK14304, compared with NPY+/+ littermate controls. Using electrophysiological techniques we shall investigate the NPY-/- and Y1-/- functional phenotypes assessing: 1) any alterations in Y agonist potency and sensitivity to various secretagogues; 2) changes in neurogenic responses; and 3) possible compensatory mechanisms e.g. treating Y1+/+ tissue with a Y1 receptor antagonist.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Genomics in Animal Function Initiative (GAN) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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