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Development of inducible conditional knockout mice to analyse gene function in melanocyte/keratinocyte physiology

ReferenceGAN13094
Principal Investigator / Supervisor Dr David Greenhalgh
Co-Investigators /
Co-Supervisors
Dr Malcolm Briden Hodgins
Institution University of Glasgow
DepartmentInstitute of Cancer Studies
Funding typeResearch
Value (£) 274,444
StatusCompleted
TypeResearch Grant
Start date 01/11/2000
End date 01/07/2004
Duration44 months

Abstract

The function of melanocytes to produce and deliver melanin to keratinocytes depends on intimate cell-cell interactions within the epidermal melanin unit. Recent studies show that keratinocyte derived factors dominate the melanocyte environment. However, in vivo, the precise nature of these interactions remain unknown. A transgenic model of cutaneous melanocyte-specific knockout mice will be developed to address this problem by exploiting tyrosinase targeting specificity coupled to an inducible cre/loxP recombinase gene switch. This study aims to identify cell-cell factors critical for normal melanocyte physiology via deregulation of growth factor responses (c-kit, FGFR-1) and keratinocyte adhesion interactions (E-cadherin).

Summary

unavailable
Committee Closed Committee - Animal Sciences (AS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Genomics in Animal Function Initiative (GAN) [1998]
Funding SchemeX – not Funded via a specific Funding Scheme
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