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Determining the in vivo role of PLC-gamma2 using gene targeting technology
Reference
GAN13054
Principal Investigator / Supervisor
Dr Martin Turner
Co-Investigators /
Co-Supervisors
Institution
Babraham Institute
Department
Babraham Institute Department
Funding type
Research
Value (£)
207,708
Status
Completed
Type
Research Grant
Start date
29/01/2001
End date
28/08/2004
Duration
43 months
Abstract
Phospholipase C-gamma 2 (PLC-gamma 2), has been implicated in over 100 ligand activated signal transduction pathways in vitro. It is now essential to demonstrate that PLC-gamma 2 plays a physiological role in the context of the whole animal. This critical next phase requires the germline manipulation that we propose here. A gene-targeting vector has been created using information obtained from genome sequencing and will be used to generate mouse Embryonic Stem cells in which one PLC-gamma 2 allele has been disrupted. These in turn will be used to establish the mutation in the germline. We propose extensive analysis of the mutant mice by combining molecular techniques with biochemistry and cellular immunology. Furthermore we have established a collaborative network that will permit a high return of information in other physiological systems.
Summary
unavailable
Committee
Closed Committee - Animal Sciences (AS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Genomics in Animal Function Initiative (GAN) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
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