BBSRC Portfolio Analyser
Award details
Discovering the roles and interactions of proteins implicated in 5`-3`resection during DNA double -strand break repair
Reference
G20296
Principal Investigator / Supervisor
Professor Alastair Goldman
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Molecular Biology and Biotechnology
Funding type
Research
Value (£)
255,937
Status
Completed
Type
Research Grant
Start date
02/02/2004
End date
01/01/2008
Duration
47 months
Abstract
Yeast deleted for, or with mutations in, candidate genes will be tested for ability to resect DNA at a double-strand break created by the nuclease VDE (VDE-DSB). Unlike Spo11-DSBs, the VDE-DSB does not have Spo11p attached to the ends, avoiding the need to remove Spo11p to initiate resection. It will be determined which proteins are phosphorylated during wildtype meiosis. The dependency of phosphorylation on Tel1p will be tested. The significance of other candidate regulatory proteins, eg pachytene checkpoint proteins, will be tested. Interactions between possible catalytic and regulatory proteins will be assayed by co-IP. Chromatin-IP will be used to compare which proteins associate with VDE- and Spo11-DSB to initiate and proceed with resection.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search