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Development of RNAi tools for functional analysis and validation of extracellular matrix targets for resistance to plant nematodes
Reference
G19482
Principal Investigator / Supervisor
Professor Michael McPherson
Co-Investigators /
Co-Supervisors
Institution
University of Leeds
Department
Plant Biochemistry & Biotechnology
Funding type
Research
Value (£)
222,868
Status
Completed
Type
Research Grant
Start date
01/10/2003
End date
30/09/2006
Duration
36 months
Abstract
Plant nematode gene function can be assessed by RNAi techniques. We have shown effective prolonged effects of a single pre-infection RNAi treatment on establishment, development and fecundity of M.incognita. Our RNAi target was dual oxidase, shown in C. elegans to be involved in tyrosine cross- linking in the extracellular matrix (ECM). This establishes the ECM as a potent target for resistance strategies. ECM biosynthesis is critical during the moulting stages in maturation of plant nematodes. We will test different dsRNA constructs for efficacy by in planta infection assays to guide development of transgenic plants expressing effective RNAi constructs. We will test further ECM-related genes for their efficacy in disrupting nematode development. These studies will provide further understanding of ECM processes in plant nematodes.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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