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Control of alternative splicing at a 'dead end' GA5' splice site in the Fibroblast Growth Factor Receptor genes
Reference
G19433
Principal Investigator / Supervisor
Professor Gavin Screaton
Co-Investigators /
Co-Supervisors
Dr Simon Brackenridge
Institution
University of Oxford
Department
Clinical Medicine
Funding type
Research
Value (£)
194,000
Status
Completed
Type
Research Grant
Start date
01/04/2003
End date
31/03/2006
Duration
36 months
Abstract
We previously characterised a conserved, atypical 5 splice site (ACA/GAAAGT) present in vertebrate Fibroblast Growth Factor Receptor (FGFR) genes. Our results suggest a novel mechanism for competition between this splice site and a conventional (/GT) splice site present 6 nt upstream. In addition, efficient use of the human FGFR1 GA splice site is controlled by two discrete intronic elements. Using a combination of tissue culture and in vitro splicing assays, we propose to investigate further the mechanisms by which these elements (and sequences from other FGFR genes) promote use of the GA splice site, and to investigate the effects of the enhancers on the stability of U1 and U6 snRNPs bound to the pre-mRNA.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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