Award details

siRNA: a new technology for investigating gene function in developing organs

ReferenceG19227
Principal Investigator / Supervisor Professor Jamie Davies
Co-Investigators /
Co-Supervisors
Institution University of Edinburgh
DepartmentBiomedical Sciences
Funding typeResearch
Value (£) 177,692
StatusCompleted
TypeResearch Grant
Start date 01/10/2003
End date 30/09/2006
Duration36 months

Abstract

We wish to characterise and improve a new technology for manipulating selected genes during organ development in culture. The technology, using siRNAs, offers ethical, technical and economic advantages over transgenic mice. We have pilot data that confirms its effectiveness at repressing the expression of the transcription factor WT1 in kidney development. We now wish to further characterise the ability of multiple siRNAs to work simultaneously, to enter epithelia, and to pass from cell to cell in organ culture. The outcome will be a well-characterised technique offering great advantages over current technologies, and which is likely to be directly applicable to the development of many mammalian organs and body parts.

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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