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The mechanisms of microevolution of cis-acting control of animal development
Reference
G19219
Principal Investigator / Supervisor
Professor John Brookfield
Co-Investigators /
Co-Supervisors
Institution
University of Nottingham
Department
School of Molecular Medical Sciences
Funding type
Research
Value (£)
176,896
Status
Completed
Type
Research Grant
Start date
01/12/2003
End date
30/11/2006
Duration
36 months
Abstract
Enhancer sequences from developmentally important genes hairy, Ultrabithorax and Kruppel will be sequenced from the Drosophila melanogaster species group. From these sequences, we will observe the changes to binding sites for transcription factors, and investigate the microevolutionary process, including observation of whether diverged binding sites can still bind transcription factors in vitro, by gel retardation and footprinting experiments. We will also examine the expression of a beta-galactosidase transgene in D.melanogaster and in D. erecta driven by enchancer sequences from these species, and artificially generated ancestral enhancer sequences duplicating those at nodes separating the species of the melanogaster group.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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