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Development of regulated RNA aptamers as tools for in vivo post-genomic analysis

ReferenceG19036
Principal Investigator / Supervisor Professor Peter Stockley
Co-Investigators /
Co-Supervisors
Professor Adam Nelson
Institution University of Leeds
DepartmentInst of Molecular & Cellular Biology
Funding typeResearch
Value (£) 109,580
StatusCompleted
TypeResearch Grant
Start date 01/04/2003
End date 01/10/2004
Duration18 months

Abstract

We propose to demonstrate the potential of small molecular weight effectors based on aminoglycosides, coupled to RNA aptamers directed against cellular protein targets, for the dissection of complex metabolic pathways in vivo. As a test of this approach, we will use the transcription factors controlling amino acid biosynthesis in E. coli for methionine (MetJ), arginine (ArgR) and tryptophan (TrpR). The results will be assessed using gene arrays.

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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