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Histone modification and gene silencing in embryonic stem cells

Reference	G18987
Principal Investigator / Supervisor	Professor Bryan Turner
Co-Investigators / Co-Supervisors	Dr Laura O'Neill
Institution	University of Birmingham
Department	Medical Sciences - Medicine
Funding type	Research
Value (£)	279,256
Status	Completed
Type	Research Grant
Start date	01/07/2003
End date	31/12/2006
Duration	42 months

Abstract

We will investigate the relationship between acetylation and methylation of core histones and developmentally-regulated gene silencing, using the silencing of X-linked genes in female embryonic stem (ES) cells as a model system. We will raise antisera that discriminate histone isoforms mono-, di- and tri- methylated at selected lysines. ChIP assays will define patterns of histone modification on X-linked genes, centric heterochromatin and across the crucial Xist/Tsix region. We will establish when and where these patterns are put in place during differentiation. This will allow future experiments to explore protein and RNA binding by appropriately modified histone tails and the effects of deleting DNA regions targeted for modification.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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