BBSRC Portfolio Analyser
Award details
The role of RSC in assembly of specific protein -DNA complexes
Reference
G18800
Principal Investigator / Supervisor
Professor James Murray
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Institute of Biotechnology
Funding type
Research
Value (£)
196,284
Status
Completed
Type
Research Grant
Start date
01/08/2003
End date
31/07/2006
Duration
36 months
Abstract
Yeast RSC is an abundant and essential multisubunit ATP- dependent chromatin remodelling complex that modulates chromatin structure. However little is known of its specific roles and nothing of its participation in the assembly of protein-DNA complexes. RSC exists as two alternative forms containing Rsc1 or Rsc2. We have recently shown that RSc2 is necessary for the correct chromatin structure and function of STB, part of the partitioning system of 2 mu plasmid, whereas both RSc1 and RSc2 are required for centromere function. We propose a detailed analysis of the components of STB complexes and the role of RSC in their assembly using intra-nuclear DNA and protein localisation, chromatin immunoprecipation, indirect end labelling and FRET.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search