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A molecular analysis of RNA pseudoknot-dependent termination codon readthrough
Reference
G18715
Principal Investigator / Supervisor
Professor Ian Brierley
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Pathology
Funding type
Research
Value (£)
251,116
Status
Completed
Type
Research Grant
Start date
01/01/2003
End date
31/12/2005
Duration
36 months
Abstract
This project will investigate the RNA pseudoknot-dependent translational readthrough signal of murine leukemia virus (MuLV). The aim is to determine whether the pseudoknot binds to a cellular protein that is essential for stop codon suppression. We will approach this question in several ways, including supplementation of translation extracts non-functional in readthrough with fractions from an active system, a screen for binding proteins using the yeast three hybrid technique and site-specific RNA-protein crosslinking. Relevance to readthrough will be assessed by biochemical and genetic analysis. The readthrough signal represents an excellent opportunity to probe ribosome function and ribosome-mRNA interactions.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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