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Identification of in vivo targets of plant GATA transcription factors

Reference	G18363
Principal Investigator / Supervisor	Professor Philip Gilmartin
Co-Investigators / Co-Supervisors	Professor Noel Buckley, Dr Paul Devlin, Professor David Westhead
Institution	University of Leeds
Department	Plant Biochemistry & Biotechnology
Funding type	Research
Value (£)	407,660
Status	Completed
Type	Research Grant
Start date	17/02/2003
End date	17/02/2006
Duration	36 months

Abstract

The role of plant GATA factors in light-regulated gene expression has been demonstrated by in vitro analysis of nuclear GATA- binding proteins and in vivo analysis of their target sites from light- responsive genes. Our work on the Arabidopsis family of zinc finger proteins that share homology to animal and fungal GATA factors shows that members of this family can transactivate gene expression in vivo through GATA elements from in light- responsive promoters and emerging evidence from microarray analyses indicates that GATA-2 and GATA-4 are phytochrome regulated. We propose to use chromatin immuno precipitation, bioinformatics, microarrays, and in vivo imaging of promoter activity, together with available GATA miss-expression and knock- out lines to identify targets of GATA-2 and GATA-4 and initiate analyses of the transcriptional networks controlled by them.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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