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Establishing regulatory links in the control of actinorhodin production in Streptomyces coelicolor

Reference	G18095
Principal Investigator / Supervisor	Professor S Baumberg
Co-Investigators / Co-Supervisors	Dr Kenneth McDowall
Institution	University of Leeds
Department	Inst of Integrative & Comparative Biolog
Funding type	Research
Value (£)	195,196
Status	Completed
Type	Research Grant
Start date	15/02/2003
End date	15/02/2006
Duration	36 months

Abstract

Our understanding of the cascades that regulate the expression of pathway-specific activators of secondary metabolites is far from complete. Using biochemical approaches we have found, characterised and partially purified a factor that binds specifically to sites flanking the promoter of actII-ORF4. We now propose to (i) complete the identification of the specific DNA-binding factor and then (ii) create a knockout of the corresponding gene and study the phenotypic effects. In complementary experiments, we will also (iii) identify important nucleoside contacts within the binding sites and then determine the consequence of their mutation, comparing with results of (ii). Finally, we plan to (iv) identify other components in the regulation of actII-ORF4 and other possible targets for the specific DNA-binding factor.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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