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Parasexual genetics for Dictyostelium molecular biology
Reference
G17939
Principal Investigator / Supervisor
Professor Robert Insall
Co-Investigators /
Co-Supervisors
Institution
University of Birmingham
Department
Sch of Biosciences
Funding type
Research
Value (£)
203,292
Status
Completed
Type
Research Grant
Start date
01/05/2003
End date
31/10/2006
Duration
42 months
Abstract
We will establish genetic tools, based around parasexual genetics, which will greatly enhance the experimental tractability of Dictyostelium. We will generate double, triple and more complex mutants, using mitotic recombination for linked genes; set up diploid- based methods for handling disruptions in essential genes; and makes new strains to extend the usefulness of these techniques. We will use these tools to address two hitherto intractable problems. Firstly we make multiple Ras knockouts, using existing single mutants, to test the redundancy and promiscuity of Dictyostelium Ras pathways. Secondly, we will create various gene replacements to allow analysis of the essential actin binding protein Arp2.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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