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A transgenic approach to investigate the developmental role of an evolutionarily conserved RNA binding protein
Reference
G17920
Principal Investigator / Supervisor
Professor David Elliott
Co-Investigators /
Co-Supervisors
Institution
Newcastle University
Department
School of Cell and Molecular Biosciences
Funding type
Research
Value (£)
184,172
Status
Completed
Type
Research Grant
Start date
10/03/2003
End date
10/03/2006
Duration
36 months
Abstract
Based on its conservation in all mammals, the RNA binding protein hnRNPG-T is most likely an important regulator of RNA processing networks during meiosis. We will test this hypothesis in transgenic mice by replacing the Hnrnpgt gene with a Green Fluorescent Protein- PGK-neo cassette. Germ cell development will be monitored in mice heterozygous and homozygous for this null allele. Patterns of gene expression will be monitored by microarray analysis from RNA purified from cells using fluorescence activated cell sorting. RNA from cells with and without a functional hnRNPG-T protein will be used as a resource to identify direct molecular targets and genetic pathways controlled by hnRNPG-T during meiosis.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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