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Regulation of nucleosome sliding by ISWI chromatin remodeling complexes in vivo

ReferenceG17914
Principal Investigator / Supervisor Dr E Mellor
Co-Investigators /
Co-Supervisors
Institution University of Oxford
DepartmentBiochemistry
Funding typeResearch
Value (£) 242,876
StatusCompleted
TypeResearch Grant
Start date 01/03/2003
End date 28/02/2006
Duration36 months

Abstract

In yeast, the imitation switch (ISWI) chromatin remodelling complexes are required to repress CLB2 and SWI5 during the cell cycle by sliding nucleosomes and displacing TBP1. At CLB2 and SWI5, distinct ATPases within the ISWI complexes are involved in repression of transcription. The remodelling activities of these enzymes are regulated by DNA-binding Forkhead transcription factors. Using chromatin immunoprecipitation and in vivo footprinting of nucleosome positions we aim (i) to characterise the particular ATPases involved in regulating CLB2 and SWI5, (ii) establish how the ATPases are targeted to specific nucleosomes and (iii) define how the activity of the ATPases is regulated during the cell cycle by the Forkhead factors.

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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