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Regulation of nucleosome sliding by ISWI chromatin remodeling complexes in vivo
Reference
G17914
Principal Investigator / Supervisor
Dr E Mellor
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Biochemistry
Funding type
Research
Value (£)
242,876
Status
Completed
Type
Research Grant
Start date
01/03/2003
End date
28/02/2006
Duration
36 months
Abstract
In yeast, the imitation switch (ISWI) chromatin remodelling complexes are required to repress CLB2 and SWI5 during the cell cycle by sliding nucleosomes and displacing TBP1. At CLB2 and SWI5, distinct ATPases within the ISWI complexes are involved in repression of transcription. The remodelling activities of these enzymes are regulated by DNA-binding Forkhead transcription factors. Using chromatin immunoprecipitation and in vivo footprinting of nucleosome positions we aim (i) to characterise the particular ATPases involved in regulating CLB2 and SWI5, (ii) establish how the ATPases are targeted to specific nucleosomes and (iii) define how the activity of the ATPases is regulated during the cell cycle by the Forkhead factors.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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