Award details

Genes and proteins of plasmid movement in Streptomyces

Reference	G16018
Principal Investigator / Supervisor	Professor S Baumberg
Co-Investigators / Co-Supervisors	Professor Peter Henderson, Dr Hilary Richards, Professor John Ward
Institution	University of Leeds
Department	Inst of Integrative & Comparative Biolog
Funding type	Research
Value (£)	194,716
Status	Completed
Type	Research Grant
Start date	07/01/2002
End date	06/11/2005
Duration	46 months

Abstract

Conjugative plasmids mediating DNA transfer are common in streptomycetes, but little is known about the functions of conjugation proteins such as those of plasmid pIJ101:Tra (for transfer) and SpdAB/KilB (for spread of plasmid through the recipient mycelium). Pilot experiments with SpdA have demonstrated expression in E. coli. This project will go on to study the association of each expressed protein with membranes, plasmid DNA, and other proteins. Alongside biochemistry, genetics will be used to answer questions focussed on spread, including: is Tra needed for spread? What is the DNA specificity of SpdAB/Kil? The outcome will be i) enhanced understanding of natural gene transfer and (ii) a greater ability to manipulate these important cloning vectors.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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