Award details

Functional analysis of inversin a major left-right axis determinant

Reference	G16015
Principal Investigator / Supervisor	Professor Tom Strachan
Co-Investigators / Co-Supervisors	Professor Judith Goodship
Institution	Newcastle University
Department	Institute of Human Genetics
Funding type	Research
Value (£)	178,272
Status	Completed
Type	Research Grant
Start date	01/09/2001
End date	01/09/2004
Duration	36 months

Abstract

The inversin gene; the locus for situs inversus and pancreatic and renal abnormalities in the inv mouse, has been cloned. Follow-up evolutionary screens showed strong conservation of an ankyrin repeat region, and a downstream 50 a.a. region. Inversin-specific antibody has been successfully raised and preliminary expression studies show a highly dynamic expression pattern, including centrosomal association. Calmodulin has been shown to bind inversin and other potential protein partners including ACP2, an anaphase promoting complex protein, and MAD2, a mitotic checkpoint protein have been identified. This project will validate potential protein partners, carry out detailed intracellular localisation studies (including studies of embryonic node and renal epithelial cells) and carry out a microarray screen of altered gene expression immediately consequent upon the kidney phenotype.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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