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Novel engineered G-quadruplex binding proteins for the inhibition of telomerase function and telomere length regulation
Reference
G15867
Principal Investigator / Supervisor
Professor Sir Shankar Balasubramanian
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Chemistry
Funding type
Research
Value (£)
157,148
Status
Completed
Type
Research Grant
Start date
01/04/2002
End date
31/08/2005
Duration
41 months
Abstract
The proposed work will build on recently published observations and aims to generate engineered zinc finger proteins that bind to telomeric G-quadruplex DNA with high affinity and specificity. The approach will be partially based on selection from a phage library, but will also employ rational protein minimisation/optimisation strategies. The best candidate proteins are expected to possess a binding activity that can interfere with the processing and synthesis of telomeric DNA, by inhibition of telomerase or other mechanisms. Various hypotheses for telomere length regulation will be tested both in-vitro and in cell-based studies using these novel probes. Evidence will also be sought for the existence of the G-quadruplex structure in cells.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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