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A genetic analysis of links between nuclear structure and function in vertebrate cells

Reference	G15592
Principal Investigator / Supervisor	Professor Dean Jackson
Co-Investigators / Co-Supervisors	Professor Stuart Wilson
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	307,840
Status	Completed
Type	Research Grant
Start date	01/03/2002
End date	11/07/2005
Duration	40 months

Abstract

In vertebrate cells, the preservation of genomic integrity requires that DNA synthesis is performed with extreme precision. Using chicken DT40 cells as a vertebrate model system we will generate conditional gene knockouts of two genes that are essential for nuclear structure and DNA synthesis; lamin B and PCNA. Gene disruptions will be complemented with mutated and tagged (e.g. GFP or flag-tagged) gene products to study the anatomy and dynamic behaviour of replication factories and DNA foci in both living and extracted cells. We believe that this robust yet versatile genetic approach is essential to evaluate epigenetic features that influence chromatin function in higher eukaryotes.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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