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Interactions between RNA polymerase and a transcription-repair coupling factor
Reference
G15249
Principal Investigator / Supervisor
Professor Nigel Savery
Co-Investigators /
Co-Supervisors
Institution
University of Bristol
Department
Biochemistry
Funding type
Research
Value (£)
167,828
Status
Completed
Type
Research Grant
Start date
01/10/2001
End date
01/10/2004
Duration
36 months
Abstract
The E. coli Mfd protein is a transcription-repair coupling factor, responsible for promoting the preferential repair of DNA lesions in the template strand of expressed genes. Its role is to remove stalled RNA polymerase (RNAP) from sites of DNA damage and to recruit the UvrABC repair apparatus. Tis project will quantify the RNAP-binding and disassociation activities of Mfd by in vitro analysis of the purified proteins; identify the residues within Mfd and RNAP that are required for their interaction by in vivo genetic screening; and determine the mechanistic consequences of mutating those residues. This will provide insights into the mechanisms of transcription-coupled repair, transcription termination and RNAP function.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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