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Interactions of E. coli MelR protein

ReferenceG15075
Principal Investigator / Supervisor Professor Steve Busby
Co-Investigators /
Co-Supervisors
Dr Eva Hyde
Institution University of Birmingham
DepartmentSch of Biosciences
Funding typeResearch
Value (£) 209,528
StatusCompleted
TypeResearch Grant
Start date 01/11/2001
End date 30/11/2004
Duration37 months

Abstract

The Escherichia coli MelR protein is a typical member of the AraC family of transcription factors. We propose to investigate the role of the MelR N-terminal melibioise-binding domain in MelR-dependent transcription activation. In order to understand the mechanism of MelR-dependent transcription activation, we will identify the interactions between MelR and the different subunits of RNA polymerase holoenzyme. Finally, in recent work, we have shown that, at the melAB promoter, the cyclic AMP receptor protein is recruited by direct interaction with MelR. We propose to investigate the molecular details of this interaction.

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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