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The role of chromatin structure and DNA methylation in transcriptional repression by (CGG)n repeats
Reference
G14978
Principal Investigator / Supervisor
Professor Matt Guille
Co-Investigators /
Co-Supervisors
Dr Alan Thorne
Institution
University of Portsmouth
Department
Sch of Biological Sciences
Funding type
Research
Value (£)
180,160
Status
Completed
Type
Research Grant
Start date
16/07/2001
End date
15/02/2006
Duration
55 months
Abstract
The expansion of a (CGG)n triplet repeat sequence in the FMR1 gene can lead to Fragile X syndrome by a mechanism involving transcription repression. Preliminary experiments show that repression is mimicked on templates injected into Xenopus oocytes. Oocytes assemble templates into chromatin and contain all the eukaryotic transcription repression machinery. The effect of increasing triplet repeat length on transcription and chromatin assembly in oocytes will be tested, as will the effect of template methylation, processes known to be involved in FMR1 silencing. The data obtained will enhance our knowledge of the role of chromatin structure in Fragile X and aid development of models for testing the mechanisms of other triplet repeat diseases.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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