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Toxoplasma gondii: A model system for using microarrayed cDNAs to investigate host-intracellular pathogen relations

Reference	G14620
Principal Investigator / Supervisor	Dr James Ajioka
Co-Investigators / Co-Supervisors	Dr Richard Clarkson
Institution	University of Cambridge
Department	Pathology
Funding type	Research
Value (£)	339,764
Status	Completed
Type	Research Grant
Start date	02/04/2001
End date	01/11/2004
Duration	43 months

Abstract

A microarray system will be developed to analyse host-intracellular pathogen relationships using human host cell infection with Toxoplasma gondii as the model. Using a total RNA prep from infected host cells as a probe, the phylogenetic distance/DNA divergence between host and parasite will differentiate respective mRNAs on a single microarray. The system will be used to investigate host cell protection from apoptosis by infection with T. gondii. Host genes that show patterns of co-regulation will provide insight into the hypothetical mechanisms of protection and T. gondii gene(s) that support these hypotheses will be assessed by analysis of complete cDNA sequence and promoter analysis of upstream sequence of co-regulated genes.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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