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Chromatin acetylation and plant gene expression
Reference
G14595
Principal Investigator / Supervisor
Professor John Gray
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Plant Sciences
Funding type
Research
Value (£)
262,040
Status
Completed
Type
Research Grant
Start date
01/07/2001
End date
01/07/2004
Duration
36 months
Abstract
The aim of the project is to determine the relationship between the acetylation of histones and HMG proteins and the expression of the pea plastocyanin gene (PetE). The acetylation status of histones and HMG proteins associated with the enhancer and promoter regions of PetE will be determined by chromatin immunoprecipitation and PCR. The effect of histone deacetylase inhibitors on PetE expression will be examined by nuclear run-on assays. The association of a novel small bromodomain protein with acetylated histones and HMG proteins will be examined by chromatin immunoprecipitation. The effect of altered levels of the bromodomain protein on the expression of PetE will be examined in transgenic plants.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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